Using Lab Pro With Graphing Calculator
1) Attach the sensors, beginning with Channel 1
Channel 1 pH
Channel 2 Conductivity (dissolved solids)
Channel 3 Temperature
Channel 4 Turbidity or nitrates or calcium or dissolved oxygen or
flow rate
2) Turn on calculator
3) Select APPS key on calculator
4) Select Datamate
5) Let it autodetect the sensors and you will see the channel
number and the names of the sensors with the readings (data)
6) Take readings and record data on table.
7) If it does not autodetect, then select 1 (Set-Up) and select
channel, and choose sensor (If conductivity use the 0-2000
setting.) and the channel will have the sensor set up. Repeat for
other sensors that are not autodetect.
Special notes:
pH sensor- Be very careful with solution. Unscrew cap before
removing sensor. Cover top with Parafilm. Then be careful with
the solution. Rinse the sensor and take reading. Rinse sensor and
return to solution. Be sure to unscrew the bottle before you push
the sensor in. Also be sure that the top is screwed on well. Be
sure it is not leaking. Place the sensor into a separate plastic
bag just in case it leaks.
If necessary:
Calibration via Y Intercept Method-
Go to Set up (number 1) select the channel with the pH sensor,
press 2 for calibration, press 3 for manual entry, and enter Y
intercept that is labeled on the sensor.
Turbidity Sensor- Be careful with the bottles. Do not empty the
cloudy standard bottle. Make sure the outside is dry and the
triangle match.
Calibration:
First Point-
Fill blank cell with distilled water, and place into the sensor.
Go to set up (number 1) locate the channel with the turbidity
sensor and press 2 which is calibrate. Once the display is
visible enter 0 for the first point.
Second point-
Place standard cell (cloudy solution) into sensor. Enter 100 for
second point.
It will return to channel display. Press 1 for OK and your
turbidity sensor will be calibrated.
Trace Metals with Hach DR2010:
Al
Ascorbic acid
Aluver 3 Al Reagent Powder Pillows
Bleaching 3 Powder Pillows
(Standard if Needed)
Fe
Ferrover Iron Reagent Powder Pillows
Cr
Hot Plate with pot
Cr 1 Reagent 1 Pillows
Cr 2 Reagent 1 Pillows
Acid Reagent Powder Pillows
ChromaVer 3 Chromium Reagent Powders
Zn
Cyclohexanone
Zinocover 5 Reagent Powder Pillows
Graduated cylinders
Ni/Co
Pan Indicator
Phthalate-phosphate Reagent Powder Pillows
EDTA
Reagent Powder Pillows
Cu
Big Beaker
Cuver 1 Copper Reagent powder pillows
bottle dionized water
Lead and Cu with the HSA 1000
Put tablet in the cap of the bottle and crush it.
Fill tube with sample water.
Add powder and shake to dissolve.
Press on.
Select test Pb or Cu.
Be sure to check calibration code with code on bottom of test
box.
Hit enter.
Hit enter.
When it says to insert electrode do so. Be careful not to touch
and to insert it right the first time. There is no second chance.
Hit enter.
Place tube in weighed holder. Gently immerse the electrode into
the solution.
Watch and wait for the answer to display. Write on special data
paper.
DR2010
&
HAS 100
Heavy Metal Test
Aluminum
Click here for a step by step demonstration with pictures
1) Enter 10 for aluminum, then the display will show Dial nm to522, if it does not show 522 then rotate dial to 522. Once correct wavelenght is entered the display will show Zero Sample then mg/L Al3
2) Fill a 50mL cylinder with the sample water to the 50 mL mark.
3) Add one Ascobic Acid powder pillow to the sample. Cover cylinder with parafilm and shake till Ascorbic acid is fully disolved.
4) Add one AluVer 3 Alluminum Reagent powder pillow. Cover with parafilm. Dissolve.
5) Pour 25mL of sample into a 25mL sample cell.
6) Add one Bleaching 3 Reagent Powder Pillow to the remaining sample in the cylinder. Cover with
parafilm, and shake well for 30 seconds.
7) Pour the remainig 25mL of the sample from the cylinder into a 25mL sample cell. This sample will be the blank.
8) Allow for a 15 minute reaction time.
9) Within 5 minutes after the reaction time place the blank into the cell holder and close the light sheild.
10) Press: ZERO. The display will show: Zeroing . . . then : 0.00mg/L Al3.
11) Imediatly place the prepared sample into the cell holder. Close the light shield.
12) Rress: READ The result will then be displayed.
Dissolved Lead in Water (HSA100)
1) To prepare sample for test take Pb pill and crush it in cap using tool. Pour the powdered pill into a tube
and pour sample into tube up to top line. Put tube cap on tightly and shake until dissolved completly.
2) Place sample tube into tube holder with cap off.
3) Press on on HSA 100 and chose Lead, and press enter.
4) Check calibration code with the code on the bottom of the box containig the electrode, if different eneter code. Press enter.
5) The instrumnet will preform a system check.
6) The sample number will appear. Press enter.
7) The display will show Instert Electode. To insert electrode tear foil rapper along dotted line and remove
the top part of the repper. Place the electrode into the connector. Remove bottom of foil rapping without
touching electrode. Hit eneter.
8) The display will show Immerse Electrode. Immerse the electrode into the sample tube until the connector cap fits firmly onto the tube holder. The instrument will sense that the electrode is emmersed and will start sensing. After a minute the instrument the instrument will display the result of the test.
For Total recoverable lead:
1) Acid digestion is necessary. Transfer 100 mL of well-mixed, acid preserved sample to a 250-mL beaker.
2) Add 1 mL of concentrated nitric acid.
3) Add 0.5 mL of concentrated hydrocloric acid.
4) Heat the sample on a hot plate at 85 degrees.
5) Cover the sample with a watch glass. Reflux for 30 minutes.
6) Let the sample cool. Quantitativley transfer the sample to a 100-mL volumetric flask.
7) Dilute to vulome with deionized water.
8) Neutralize the sample as described in the next section.
Neutralizing
1) Rinse one 20-mL screw-capoped tube with the acidified sample. Fill the tube to the 20-mL mark with
sample.
2) Add two drops of methyl-orange. Add sodium hydroxide drop by drop untl the solution changes from
pink to yellow.Between each drop, cap the tube and shake to mix. Record the total number of drops added. The yellow color indicates the sample is neutralized.
3) Remove a 5-mL screw capped test tube from the Sensor Pack. Fill to the 5-mL mark with the neutralized
sample.
4) Procede with the Dissolve Lead in Water Test.
5) Empty the 20-mL platic tube immediatly and rinse with deionized water.
Dissolved Copper in Water (HSA100)
1) To prepare sample for test take Cu pill and crush it in cap using tool. Pour the powdered pill into a tube
and pour sample into tube up to top line. Put tube cap on tightly and shake until dissolved completly.
2) Place sample tube into tube holder with cap off.
3) Press on on HSA 100 and chose Copper, and press enter.
4) Check calibration code with the code on the bottom of the box containig the electrode, if different eneter code. Press enter.
5) The instrumnet will preform a system check.
6) The sample number will appear. Press enter.
7) The display will show Instert Electode. To insert electrode tear foil rapper along dotted line and remove
the top part of the repper. Place the electrode into the connector. Remove bottom of foil rapping without
touching electrode. Hit eneter.
8) The display will show Immerse Electrode. Immerse the electrode into the sample tube until the connector cap fits firmly onto the tube holder. The instrument will sense that the electrode is emmersed and will start sensing. After a minute the instrument the instrument will display the result of the test.
Nickel
Click here for a step by step demonstration with pictures
1) Enter 340 for
aluminum, then the display will show Dial nm to 560, if it does
not show 560 then rotate dial to 522. Once
correct wavelenght is entered the display will show Zero Sample
then mg/L Ni PAN. Then put the 10mL cell-riser.
2) Fill a 10mL with
10 mL of the sample. And Fill another 10 mL sample cell with 10mL
of deionized water (this is the
blank).
3) Add one
Phthalate Phosphate Reagent powder pillow to each sample cell.
Cover cylinder with parafilm and shake till
fully disolved.
4) Add 0.5 mL of 3%
PAN indicator solution to each cell. Cover with parafilm.
Dissolve.
5) Allow for a
fiftenn minute reaction time.
6) After fifteen
minutes add the contents one EDTA Reagent power pillow to each
cell .Cover Dissolve.
7) Put the blank
into the cell holder.
8) Press: ZERO. The
display will show: Zeroing . . . then : 0.00mg/L Ni PAN
9) Imediatly place
the prepared sample into the cell holder. Close the light shield.
10) Press: READ The
result will then be displayed.
11) to adjust for
the presence of cobalt rotate the dial to show 620nm then press
enter.
12)Place the blank
into the holder and zero it.
13) Place the
prepared sample into the holder and close the shield and press
read.
14) Use the
equation: mg/L Ni (step 12) - (0.89)*mg/L Ni (step 15) = mg/L Ni
Copper
Click here for a step by step demonstration with pictures
1.
Enter 265 then hit enter. Rotate the dial to 510 mn. The display will show Zero
sample then mg/L Fe FV.
2. Insert the cell riser into the cell holder.
3. Fill a sample cell with 10 mL of the sample.
4. Add the contents of one CuVer Copper Reagent powder pillow to the
sample cell.
5. Allow for a two minute reaction period.
6. After two minutes fill another 10 mL sample cell with 10mL of the
sample. This is the blank.
7. Place the blank into the cell holder, close the light sheild and
pres zero. The display will show Zeroing . . . then 0.00 mg/L FE FV.
8. Then place the prepared sample into the cell holder close the light
sheild and hit Read.
Cobalt
Click here for a step by step demonstration with pictures
Lead
Click here for a step by step demonstration with pictures
Zinc
Click here for a step by step demonstration with pictures