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1. Read the
introductory story, "The Farmers and the Pickle Factory"
(Attachment
#1 or http://www.classtech2000.com/biolvhs/pickle/farmpap.htm)
2. Read and study Seeds and Germination
(Attachment
#2 or http://www.classtech2000.com/biolvhs/pickle/germ/seedgerm.htm)
3. Place 2 sheets of paper towel into each of 16 resealable bags.
4. Label two bags each as
0, 5, 10, 20, 40, 60, 80, and 100 to correspond to the pickle juice
dilutions.
5. Place 10 corn seeds in
each bag of one set of 8 bags (one of each dilution) and label the bags
“Corn.”
6. Place 10 mustard seeds
in each bag of the other set of 8 bags and label the bags “Mustard.”
7. Wet the paper towel in
each bag with 10 ml of the dilution of pickle juice corresponding to its
label (e.g. 10 ml of water in bag 0, 10 ml of 5% in bag 5, etc.).
8. Do not seal the bags.
Seal only the corners to prevent evaporation.
9.
Lay the bags on the cookie tray grouped according to %pickle
juice.
10. Cover the bags with
newspaper so no light reaches the seeds.
11. After 24 hours, open each bag and count the number of seeds that
have germinated in each bag. Measure
in millimeters the length of the seedling from tip to tip using a piece
of threat or dental floss. Record
you results in the Seed Germination Table
(Attachment
#3 or
http://www.classtech2000.com/biolvhs/pickle/germtab.htm).
For each concentration determine the average total length of the
seedling. Each bag
has 10 seeds. Those seeds
that do not geminate have the length of the seed only. Record the data
for each seed and concentration on the Seed Germination Table.
12.Take pictures and record the picture number shown on the camera and
the description of the specimen in the picture.
13. Repeat steps 11 and 12 at 48 hours and 72 hours and after 7 days.
Record data each time on table provided. (Note that a different
Seed Germination Table is required for each day of data collection.)
14. On the 7th
day look at the seeds at each of the concentration of pickle juice.
Select the seedling that is the longest in total length.
The on a piece of graph paper lay out the seedlings from 0% - 100
% pickle juice. You are
making a living graph. Lay the paper long ways and make a line for the
X-axis (% Pickle Juice) Every 4 cm will represent 10% Pickle Juice.
(A picture of an example of this is available in
The Pickle "Dill"emma PowerPoint How To (Attachment
#4 or
http://www.classtech2000.com/biolvhs/pickle/ppt/pptpick.htm)).
Make makers on the paper. The
Y-axis is total length of the seedling.
Arrange the seedlings and then take a picture of your graph.
You will notice that the seeds are very crowded in the graph
between 0% and 20%. Now
make another graph and only plot 0% - 60 %.
Now you can spread out the x Axis more.
Do your best to spread out the axis. Take a picture of the living
graph. Try to zoom in as close as possible. Repeat making a living graph for each type of seed.
(Please note that adequate results can often be determined in 3 days is
there is adequate growth. If
there is adequate growth, you can take the pictures for the living graph
on the third day, and the lesson takes less time.)
15. Use MS PowerPoint to
present an analysis of the data you obtain so that you can make a final
report to the farmers and pickle factory managers.
Use The Pickle "Dill"emma PowerPoint How To (Attachment
#4 or http://www.classtech2000.com/biolvhs/pickle/ppt/pptpick.htm)
to create your PowerPoint presentation. Include the following Slides:
Title Slide
Summary of Problem
Description of Investigation Procedure
Graph of total lengths and number germinated vs. Pickle Juice
Concentration Results
Picture of Living Graph with Caption.
Conclusion/Recommendation to farmers (Including how citizens can help
solve or prevent the problem)
Attachment 7
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